Human RP105 monoclonal antibody enhances antigen-specific antibody production in unique culture conditions

Human RP105 monoclonal antibody enhances antigen-specific antibody production in unique culture conditions

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Summary: Detecting antibodies, particularly those targeting donor human leukocyte antigens in organ transplantation and self-antigens in autoimmune diseases, is crucial for diagnosis anodized pearl price xbox and therapy.Radioprotective 105 (RP105), a Toll-like receptor family protein, is expressed in immune-competent cells, such as B cells.Studies in mice have shown that the anti-mouse RP105 antibody strongly activates B cells and triggers an adjuvant effect against viral infections.However, the anti-human RP105 antibody (ɑhRP105) weakly activates human B cells.This study established new culture conditions under, which human B cells are strongly activated by the ɑhRP105.

When combined with CpGDNA, specific antibody production against blood group carbohydrates, ɑGal, and SARS-CoV-2 was successfully detected in human B cell cultures.Furthermore, comprehensive analysis using liquid chromatography-electrospray ionization tandem mass spectrometry, single-cell RNA sequencing, and quantitative real-time PCR revealed that ɑhRP105 triggered a poise pads in bulk different activation stimulus compared to CpGDNA.These findings could help identify antibody-producing B cells in cases of transplant rejection and autoimmune diseases.